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This channel design consists of two layers: The bottom one is a polydimethylsiloxane (PDMS) plate with regular cubic pockets. These pockets are filled with. For iGEM , teams will participate in a two-tiered competition. Regional Jamborees will be held in October and the World Championship. Material and methods � Double digest for cloning � Ligation � Transformation � PCR � Colony PCR � Preparation of glycerol stocks � AlcR testing � Chemically competent E.

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For proof of concept, we engineered E. To show that our system can be activated by different signals, as a proof of principle, we modified the circuit so that it can be induced by arabinose. Our lab efforts were supported by models in both the direct and the indirect sun light detection strategy as well as in the PABA production. This means that even upon parameter variation, the GFP band does not dissapear, just gets shifted or varies in thickness.